《植物生理学报》 2015, 51(5): 792-796

基于核磁共振的水稻根细胞内pH值的测定方法

刘于^*

浙江大学生命科学学院, 杭州310058

通信作者：刘；E-mail: liuyu_zju@zju.edu.cn；Tel: 0571-88206177

摘　要：

采用³¹P-核磁共振技术(³¹P-NMR)建立了无损伤测定水稻根系细胞内pH值的方法。通过测定水稻根系细胞内磷酸盐化学位移间接确定细胞液泡及细胞质内pH, 计算得到正常水培条件下水稻根系细胞液泡及细胞质内pH分别为5.43±0.05和7.45±0.04。另外, 用该方法还测定了磷饥饿条件下水稻根细胞内pH的变化。

关键词：³¹P-核磁共振; 水稻根; 细胞pH值

收稿：2015-03-09   修定：2015-04-27

资助：国家自然科学基金项目(31400224)和浙江大学实验技术研究项目(2013-15)。 致谢 浙江大学生命科学学院植物生理学与生物化学国家重点实验室吴平教授(已故)帮助建立NMR测试平台。

NMR-Based Measurement of Intracellular pH in Rice Roots

LIU Yu^*

College of Life Sciences, Zhejiang University, Hangzhou 310058, China

Corresponding author: LIU Yu; E-mail: liuyu_zju@zju.edu.cn; Tel: 0571-88206177

Abstract:

A ³¹P nuclear magnetic resonance (³¹P-NMR) method to measure noninvasively intracellular pH in rice roots was described in this paper. The cytoplasmic and vacuolar pH values (pHc and pHv) were estimated indirectly from the chemical shift of phosphate (Pi) present in the cytoplasm and vacuole of rice roots. Using the ³¹P-NMR method, pHc and pHv of the rice roots in hydroponic culture were obtained at 5.43±0.05 and 7.45±0.04, respectively. And, changes of intracellular pH in rice root under phosphorus starvation condition were also determined.

Key words: ³¹P-NMR; rice roots; intracellular pH